DNA extraction leads to bias in bacterial quantification by qPCR

Quantitative PCR (qPCR) has become a widely used technique for bacterial quantification. The affordability, ease of experimental design, reproducibility, and robustness of qPCR experiments contribute to its success. The establishment of guidelines for minimum information for publication of qPCR expe...

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Detalhes bibliográficos
Autor principal: Lima, Ângela Martins (author)
Outros Autores: França, Ângela Maria Oliveira Sousa (author), Muzny, Christina A. (author), Taylor, Christopher M. (author), Cerca, Nuno (author)
Formato: article
Idioma:eng
Publicado em: 2022
Assuntos:
Bacterial load quantification
gDNA extraction efficiency
qPCR
gDNA yield
Exogenous control
Calibration curve
qPCR reaction efficiency
Science & Technology
Texto completo:https://hdl.handle.net/1822/81084
País:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/81084

Texto Completo

https://hdl.handle.net/1822/81084

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