DNA extraction leads to bias in bacterial quantification by qPCR

Quantitative PCR (qPCR) has become a widely used technique for bacterial quantification. The affordability, ease of experimental design, reproducibility, and robustness of qPCR experiments contribute to its success. The establishment of guidelines for minimum information for publication of qPCR expe...

Full description

Bibliographic Details
Main Author: Lima, Ângela Martins (author)
Other Authors: França, Ângela Maria Oliveira Sousa (author), Muzny, Christina A. (author), Taylor, Christopher M. (author), Cerca, Nuno (author)
Format: article
Language:eng
Published: 2022
Subjects:
Bacterial load quantification
gDNA extraction efficiency
qPCR
gDNA yield
Exogenous control
Calibration curve
qPCR reaction efficiency
Science & Technology
Online Access:https://hdl.handle.net/1822/81084
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/81084

Internet

https://hdl.handle.net/1822/81084

Similar Items