Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner

TERT promoter mutations reactivate telomerase, allowing for indefinite telomere maintenance and enabling cellular immortalization. These mutations specifically recruit the multimeric ETS factor GABP, which can form two functionally independent transcription factor species: a dimer or a tetramer. We...

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Bibliographic Details
Main Author: Mancini, Andrew (author)
Other Authors: Magalhães, Ana Xavier Silva (author), Woods, Wendy S. (author), Nguyen, Kien-Thiet (author), Amen, Alexandra M. (author), Hayes, Josie L. (author), Fellmann, Christof (author), Gapinske, Michael (author), McKinney, Andrew M. (author), Hong, Chibo (author), Jones, Lindsey E. (author), Walsh, Kyle M. (author), Bell, Robert J. A. (author), Doudna, Jennifer A. (author), Costa, Bruno Marques (author), Song, Jun S. (author), Perez-Pinera, Pablo (author), Costello, Joseph F. (author)
Format: article
Language:eng
Published: 2018
Subjects:
Glioblastoma
TERT promoter mutation
GABP
Cancer immortality
Telomerase
Telomeres
Ciências Médicas::Medicina Básica
Science & Technology
Online Access:http://hdl.handle.net/1822/57876
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/57876
Description
Summary:TERT promoter mutations reactivate telomerase, allowing for indefinite telomere maintenance and enabling cellular immortalization. These mutations specifically recruit the multimeric ETS factor GABP, which can form two functionally independent transcription factor species: a dimer or a tetramer. We show that genetic disruption of GABPβ1L (β1L), a tetramer-forming isoform of GABP that is dispensable for normal development, results in TERT silencing in a TERT promoter mutation-dependent manner. Reducing TERT expression by disrupting β1L culminates in telomere loss and cell death exclusively in TERT promoter mutant cells. Orthotopic xenografting of β1L-reduced, TERT promoter mutant glioblastoma cells rendered lower tumor burden and longer overall survival in mice. These results highlight the critical role of GABPβ1L in enabling immortality in TERT promoter mutant glioblastoma.

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