Flow-FISH using nucleic acid mimic probes for the detection of bacteria

Flow-Fluorescence in situ hybridization (Flow-FISH) enables multiparametric high-throughput detection of target nucleic acid sequences at the single cell-level, allowing an accurate quantification of different cell populations by using a combination of flow cytometry and fluorescent in situ hybridiz...

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Bibliographic Details
Main Author: Azevedo, Andreia S. (author)
Other Authors: Rocha, Rui (author), Dias, Nicolina Marques (author)
Format: bookPart
Language:eng
Published: 2021
Subjects:
Peptide nucleic acid
Locked nucleic acid
2'O-methyl RNA
Nucleic acid mimics
Hybridization
Fixation
Permeabilization
Flow cytometry
Online Access:http://hdl.handle.net/1822/74496
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/74496

Internet

http://hdl.handle.net/1822/74496

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