A new cuticle scale hydrolysing protease from beauveria brongniartii

From a screening for the production of new proteases specific for cuticle scales, Beauveria brongniartii was selected producing an alkaline Ca++ dependent protease. The purified had a molecular weight of 27 kDa and a pI value of 8.0. Substrate specificities of model substrates (wool with partially r...

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Bibliographic Details
Main Author: Erlacher, Angelika (author)
Other Authors: Sousa, Fernanda (author), Schroeder, M. (author), Jus, S. (author), Kokol, V. (author), Paulo, Artur Cavaco (author), Gübitz, Georg M. (author)
Format: article
Language:eng
Published: 2006
Subjects:
Wool
Cuticle
Protease
Beauveria brongniartii
Science & Technology
Online Access:http://hdl.handle.net/1822/12954
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/12954
Description
Summary:From a screening for the production of new proteases specific for cuticle scales, Beauveria brongniartii was selected producing an alkaline Ca++ dependent protease. The purified had a molecular weight of 27 kDa and a pI value of 8.0. Substrate specificities of model substrates (wool with partially removed cuticles treated with SDS) were analyzed by protein release, dissolved organic carbon (DOC) and nitrogen analysis. The C/N ratio of released material turned out to be a good parameter to determine the site of action of proteases on fibres. Compared to other enzymes, the fungal protease preferentially hydrolyzed cuticle scales and has thus a potential for antishrinking pre-treatment of wool fabrics.

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