ClearT immersion optical clearing method for intact 3D spheroids imaging through confocal laser scanning microscopy

Spheroids are 3D in vitro platforms that fill the gap between the 2D cell cultures and animal models on the therapeutics development pipeline. Yet, the methods and equipment used in the in vitro assays are optimized for the analysis of cells cultured as monolayers. For instance, confocal laser scann...

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Bibliographic Details
Main Author: Costa, Elisabete Cristina da Rocha (author)
Other Authors: Moreira, André Ferreira (author), Diogo, Duarte Miguel de Melo (author), Correia, Ilídio Joaquim Sobreira (author)
Format: article
Language:eng
Published: 2018
Subjects:
Online Access:http://hdl.handle.net/10400.6/4735
Country:Portugal
Oai:oai:ubibliorum.ubi.pt:10400.6/4735