Elimination of glucose contamination from adipocyte glycogen extracts

Glycogen was quantified in rat adipocytes by isolation using conventional KOH digestion and ethanol precipitation, followed by hydrolysis and spectrophotometric assay of the glucose product. A concentration of 0.193 ± 0.020 [mu]mol glucosyl units/106 cells was recorded. When this procedure was modif...

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Detalhes bibliográficos
Autor principal: Nunes, Patricia M. (author)
Outros Autores: Carvalho, Eugénia (author), Jones, John G. (author)
Formato: article
Idioma:eng
Publicado em: 2008
Assuntos:
Texto completo:http://hdl.handle.net/10316/5289
País:Portugal
Oai:oai:estudogeral.sib.uc.pt:10316/5289