Elimination of glucose contamination from adipocyte glycogen extracts

Glycogen was quantified in rat adipocytes by isolation using conventional KOH digestion and ethanol precipitation, followed by hydrolysis and spectrophotometric assay of the glucose product. A concentration of 0.193 ± 0.020 [mu]mol glucosyl units/106 cells was recorded. When this procedure was modif...

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Bibliographic Details
Main Author: Nunes, Patricia M. (author)
Other Authors: Carvalho, Eugénia (author), Jones, John G. (author)
Format: article
Language:eng
Published: 2008
Subjects:
Online Access:http://hdl.handle.net/10316/5289
Country:Portugal
Oai:oai:estudogeral.sib.uc.pt:10316/5289