Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses

This work is focused on FISH technique optimization for yeast and bacteria analysis, by enhancing the FISH signals by increasing the number of fluorescent cells and their fluorescence intensities. With this aim different fixation procedures and hybridization conditions were tested. The modifications...

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Detalhes bibliográficos
Autor principal: Pérez, Marina González (author)
Outros Autores: Vieira, Ricardo (author), Nunes, Patrícia (author), Pereira, António (author), Candeias, António (author), Caldeira, Ana T. (author)
Formato: article
Idioma:eng
Publicado em: 2016
Assuntos:
FISH
microorganisms
bioprocesses
Texto completo:http://hdl.handle.net/10174/17068
País:Portugal
Oai:oai:dspace.uevora.pt:10174/17068
Descrição
Resumo:This work is focused on FISH technique optimization for yeast and bacteria analysis, by enhancing the FISH signals by increasing the number of fluorescent cells and their fluorescence intensities. With this aim different fixation procedures and hybridization conditions were tested. The modifications introduced in the procedure represent an important contribution for FISH optimization.

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