Optimization of Fluorescence In Situ Hybridization technique for analyzing microorganisms involved in bioprocesses

This work is focused on FISH technique optimization for yeast and bacteria analysis, by enhancing the FISH signals by increasing the number of fluorescent cells and their fluorescence intensities. With this aim different fixation procedures and hybridization conditions were tested. The modifications...

Full description

Bibliographic Details
Main Author: Pérez, Marina González (author)
Other Authors: Vieira, Ricardo (author), Nunes, Patrícia (author), Pereira, António (author), Candeias, António (author), Caldeira, Ana T. (author)
Format: article
Language:eng
Published: 2016
Subjects:
FISH
microorganisms
bioprocesses
Online Access:http://hdl.handle.net/10174/17068
Country:Portugal
Oai:oai:dspace.uevora.pt:10174/17068
Description
Summary:This work is focused on FISH technique optimization for yeast and bacteria analysis, by enhancing the FISH signals by increasing the number of fluorescent cells and their fluorescence intensities. With this aim different fixation procedures and hybridization conditions were tested. The modifications introduced in the procedure represent an important contribution for FISH optimization.

Similar Items