Development of a short-term assay based on the evaluation of the plasma membrane integrity of the alga Pseudokirchneriella subcapitata
Membrane integrity has been used as a criterion for the definition of cell viability. In the present work, staining conditions (time and dye concentration) for the evaluation of membrane integrity in a fluorescence microplate reader, using the membrane-impermeant nucleic-acid dye SYTOX Green, were o...
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| Format: | article |
| Language: | eng |
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2012
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| Online Access: | http://hdl.handle.net/1822/21326 |
| Country: | Portugal |
| Oai: | oai:repositorium.sdum.uminho.pt:1822/21326 |