One-step chromatographic method to purify -lactalbumin from whey for nanotube synthesis purposes

A one-step anion-exchange chromatography method (NaCl gradient elution on a DEAE Sepharose Fast Flow gel column) was developed to purify -lactalbumin (-LA) from whey protein isolate. -LA nearly 100% pure (based on the total protein content) was obtained with a yield of about 39%. Besides pure -LA, w...

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Detalhes bibliográficos
Autor principal: Fuciños, C. (author)
Outros Autores: Fuciños, Pablo (author), Estévez, Natalia (author), Pastrana, Lorenzo M. (author), Vicente, A. A. (author), Rúa, María Luisa (author)
Formato: article
Idioma:eng
Publicado em: 2019
Assuntos:
Whey proteins
alfa-Lactalbumin
Purification
AEC chromatography
Nanotubes
Protein gels
α-Lactalbumin
Science & Technology
Texto completo:http://hdl.handle.net/1822/56384
País:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/56384

Texto Completo

http://hdl.handle.net/1822/56384

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