Optimizing locked nucleic acid/2-O-methyl-RNA fluorescence in situ hybridization (LNA/2OMe-FISH) procedure for bacterial detection

Despite the successful application of LNA/2OMe-FISH procedures for bacteria detection, there is a lack of knowledge on the properties that affect hybridization. Such information is crucial for the rational design of protocols. Hence, this work aimed to evaluate the effect of three essential factors...

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Detalhes bibliográficos
Autor principal: Azevedo, Andreia S. (author)
Outros Autores: Sousa, Inês M. (author), Fernandes, R. (author), Azevedo, Nuno F. (author), Almeida, Carina (author)
Formato: article
Idioma:eng
Publicado em: 2019
Assuntos:
Texto completo:http://hdl.handle.net/1822/60589
País:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/60589