Optimizing locked nucleic acid/2-O-methyl-RNA fluorescence in situ hybridization (LNA/2OMe-FISH) procedure for bacterial detection

Despite the successful application of LNA/2OMe-FISH procedures for bacteria detection, there is a lack of knowledge on the properties that affect hybridization. Such information is crucial for the rational design of protocols. Hence, this work aimed to evaluate the effect of three essential factors...

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Bibliographic Details
Main Author: Azevedo, Andreia S. (author)
Other Authors: Sousa, Inês M. (author), Fernandes, R. (author), Azevedo, Nuno F. (author), Almeida, Carina (author)
Format: article
Language:eng
Published: 2019
Subjects:
Science & Technology
Online Access:http://hdl.handle.net/1822/60589
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/60589

Internet

http://hdl.handle.net/1822/60589

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