Effects of single and combined exposures of gold (nano versus ionic form) and gemfibrozil in a liver organ culture of Sparus aurata

In vitro methods have gained rising importance in ecotoxicology due to ethical concerns. The aim of this study was to assess the single and combined in vitro effects of gold, as nanoparticle (AuNPs) and ionic (Au+) form, and the pharmaceutical gemfibrozil (GEM). Sparus aurata liver organ culture was...

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Detalhes bibliográficos
Autor principal: Barreto, A. (author)
Outros Autores: Carvalho, A. (author), Silva, D. (author), Pinto, E. (author), Almeida, A. (author), Paíga, P. (author), Correia-Sá, L. (author), Delerue-Matos, C. (author), Trindade, T. (author), Soares, A.M.V.M. (author), Hylland, K. (author), Loureiro, S. (author), Oliveira, M. (author)
Formato: article
Idioma:eng
Publicado em: 2022
Assuntos:
Fish liver culture
Gilthead seabream
NanotoxicologyIonic gold
Oxidative stress
DNA integrity
Texto completo:http://hdl.handle.net/10400.22/16622
País:Portugal
Oai:oai:recipp.ipp.pt:10400.22/16622
Descrição
Resumo:In vitro methods have gained rising importance in ecotoxicology due to ethical concerns. The aim of this study was to assess the single and combined in vitro effects of gold, as nanoparticle (AuNPs) and ionic (Au+) form, and the pharmaceutical gemfibrozil (GEM). Sparus aurata liver organ culture was exposed to gold (4 to 7200 μg·L−1), GEM (1.5 to 15,000 μg·L−1) and combination 80 μg·L−1 gold +150 μg·L−1 GEM for 24 h. Endpoints related with antioxidant status, peroxidative/genetic damage were assessed. AuNPs caused more effects than Au+, increasing catalase and glutathione reductase activities and damaging DNA and cellular membranes.

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