Immobilization of laccase on modified silica: Stabilization, thermal inactivation and kinetic behaviour in 1-ethyl-3-methylimidazolium ethylsulfate ionic liquid

Laccase was immobilized on modified silica carrier. The immobilization conditions, pH and enzyme concentration were optimized. Operational stability of 10 reaction cycles showed that immobilized laccase in buffer was stable, presenting an activity loss <30%. Nevertheless, a high decrease >80%...

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Bibliographic Details
Main Author: Ana P. M. Tavares (author)
Other Authors: Oscar Rodríguez (author), María Fernández-Fernández (author), Alberto Domínguez (author), Diego Moldes (author), María A. Sanromán (author), Eugénia A. Macedo (author)
Format: article
Language:eng
Published: 2013
Subjects:
Online Access:https://hdl.handle.net/10216/104791
Country:Portugal
Oai:oai:repositorio-aberto.up.pt:10216/104791