Plasmid DNA recovery from fermentation broths by a combined process of micro- and ultrafiltration: modeling and application

Microfiltration and ultrafiltration operations were used in tandem to isolate and purify a 6050 bp plasmid DNA (pDNA). To achieve primary isolation of the plasmid from fermentation broths, immediately after cell lysis, a 0.2 μm microfiltration membrane was selected for solid/liquid separation, which...

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Bibliographic Details
Main Author: Nunes, José C. (author)
Other Authors: Morão, A. (author), Nunes, Catherine (author), Amorim, M. T. Pessoa de (author), Escobar, I. C. (author), Queiroz, J. A. (author)
Format: article
Language:eng
Published: 2012
Subjects:
Plasmid DNA recovery
Microfiltration
Ultrafiltration
Modeling
RNA
Genomic DNA
Science & Technology
Online Access:https://hdl.handle.net/1822/21790
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/21790

Internet

https://hdl.handle.net/1822/21790

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