Characterization of social interactions and spatial arrangement of individual bacteria in multistrain or multispecies biofilm systems using nucleic acid mimics-fluorescence in situ hybridization

Biofilms are often composed of different bacterial and fungal species/strains, which form complex structures based on social interactions with each other. Fluorescence in situ hybridization (FISH) can help us identify the different species/strains present within a biofilm, and when coupled with conf...

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Bibliographic Details
Main Author: Allkja, Jontana (author)
Other Authors: Azevedo, Andreia S. (author)
Format: bookPart
Language:eng
Published: 2021
Subjects:
FISH
Nucleic acid mimics
PNA
LNA
2OMe
Bacteria
Biofilm
Multispecies
Multistrain
Microscopy
CLSM
Online Access:http://hdl.handle.net/1822/74497
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/74497
Description
Summary:Biofilms are often composed of different bacterial and fungal species/strains, which form complex structures based on social interactions with each other. Fluorescence in situ hybridization (FISH) can help us identify the different species/strains present within a biofilm, and when coupled with confocal scanning laser microscopy (CSLM), it enables the visualization of the three-dimensional (3D) structure of the biofilm and the spatial arrangement of each individual species/strain within it. In this chapter, we describe the protocol for characterizing multistrain or multispecies biofilm formation using NAM-FISH and CSLM.

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