Controlled RNA contamination and degradation and its impact on qPCR gene expression in S. epidermidis biofilms

RNA quality is of utmost importance to perform gene expression quantification by qPCR. The classical methods used to determine \RNA\ quality are based on electrophoresis and spectrophotometer assessment, namely A260/A280 and A260/A230 ratios. It was previously shown that due to the complex nature of...

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Bibliographic Details
Main Author: Carvalhais, Virgínia Maria Dinis (author)
Other Authors: Delgado-Rastrollo, M. (author), Melo, Luís D. R. (author), Cerca, Nuno (author)
Format: article
Language:eng
Published: 2013
Subjects:
S. epidermidis biofilm
RNA quality
qPCR
Impurities
emperature degradation
Temperature degradation
Science & Technology
Online Access:http://hdl.handle.net/1822/27614
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/27614

Internet

http://hdl.handle.net/1822/27614

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