Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography

The current study explores the possibility of using a polyethyleneglycol(PEG)/ammonium sulphate aqueous two-phase system (ATPS) as an early step in a process for the purification of a model 6.1 kbp plasmid DNA (pDNA) vector. Neutralised alkaline lysates were fed directly to ATPS. Conditions were sel...

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Bibliographic Details
Main Author: Trindade, Inês P. (author)
Other Authors: Diogo, Maria M. (author), Prazeres, Duarte M. F. (author), Marcos, João Carlos (author)
Format: article
Language:eng
Published: 2005
Subjects:
Aqueous two-phase systems
Liquid-liquid extraction
Plasmid DNA purification
Polyethylene glycol
Gene therapy
Ammonium sulphate
purification
plasmid
DNA
Science & Technology
Online Access:http://hdl.handle.net/1822/3424
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/3424

Internet

http://hdl.handle.net/1822/3424

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