Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography
The current study explores the possibility of using a polyethyleneglycol(PEG)/ammonium sulphate aqueous two-phase system (ATPS) as an early step in a process for the purification of a model 6.1 kbp plasmid DNA (pDNA) vector. Neutralised alkaline lysates were fed directly to ATPS. Conditions were sel...
| Autor principal: | |
|---|---|
| Outros Autores: | , , |
| Formato: | article |
| Idioma: | eng |
| Publicado em: |
2005
|
| Assuntos: | |
| Texto completo: | http://hdl.handle.net/1822/3424 |
| País: | Portugal |
| Oai: | oai:repositorium.sdum.uminho.pt:1822/3424 |