A new cloning system based on the OprI lipoprotein for the production of recombinant bacterial cell wall-derived immunogenic formulations

The conjugation of antigens with ligands of pattern recognition receptors (PRR) is emerging as a promising strategy for the modulation of specific immunity. Here, we describe a new Escherichia coli system for the cloning and expression of heterologous antigens in fusion with the OprI lipoprotein, a...

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Bibliographic Details
Main Author: Basto, Afonso P. (author)
Other Authors: Piedade, João (author), Ramalho, Ruben (author), Alves, Susana (author), Soares, Helena (author), Cornelis, Pierre (author), Martins, Carlos (author), Leitão, Alexandre (author)
Format: article
Language:eng
Published: 2013
Subjects:
African swine fever virus/genetics
Animals
Antigens, viral/genetics
Bacterial proteins/biosynthesis
Bacterial proteins/genetics
Bacterial proteins/immunology
Base sequence
Cells, cultured
Cloning, molecular/methods
Dendritic cells/immunology
Escherichia coli/genetics
Escherichia coli/metabolism
Genetic vectors/genetics
Lipoproteins/biosynthesis
Lipoproteins/genetics
Lipoproteins/immunology
Macrophages/immunology
Mice
Molecular sequence data
Pseudomonas aeruginosa/genetics
Recombinant fusion proteins/biosynthesis
Recombinant fusion proteins/genetics
Recombinant fusion proteins/immunology
Tumor necrosis factor-alpha/metabolism
Online Access:http://hdl.handle.net/10400.21/2159
Country:Portugal
Oai:oai:repositorio.ipl.pt:10400.21/2159

Internet

http://hdl.handle.net/10400.21/2159

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