Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering

Hardwood spent sulfite liquor (HSSL) is a by-product of acid sulfite pulping process that is rich in xylose, a monosaccharide that can be fermented to ethanol by Scheffersomyces stipitis. However, HSSL also contains acetic acid and lignosulfonates that are inhibitory compounds of yeast growth. The m...

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Detalhes bibliográficos
Autor principal: Pereira, Susana R (author)
Outros Autores: Sanchez i Nogue, Violeta (author), Frazao, Cludio J R (author), Serafim, Luisa S (author), Gorwa-Grauslund, Marie F (author), Xavier, Ana M R B (author)
Formato: article
Idioma:eng
Publicado em: 2017
Assuntos:
Hardwood spent sulfite liquor (HSSL)
Scheffersomyces stipitis
Lignocellulosic inhibitors
Evolutionary engineering
Bioethanol
Texto completo:http://hdl.handle.net/10773/18152
País:Portugal
Oai:oai:ria.ua.pt:10773/18152
Descrição
Resumo:Hardwood spent sulfite liquor (HSSL) is a by-product of acid sulfite pulping process that is rich in xylose, a monosaccharide that can be fermented to ethanol by Scheffersomyces stipitis. However, HSSL also contains acetic acid and lignosulfonates that are inhibitory compounds of yeast growth. The main objective of this study was the use of an evolutionary engineering strategy to obtain variants of S. stipitis with increased tolerance to HSSL inhibitors while maintaining the ability to ferment xylose to ethanol.

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