Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering

Hardwood spent sulfite liquor (HSSL) is a by-product of acid sulfite pulping process that is rich in xylose, a monosaccharide that can be fermented to ethanol by Scheffersomyces stipitis. However, HSSL also contains acetic acid and lignosulfonates that are inhibitory compounds of yeast growth. The m...

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Bibliographic Details
Main Author: Pereira, Susana R (author)
Other Authors: Sanchez i Nogue, Violeta (author), Frazao, Cludio J R (author), Serafim, Luisa S (author), Gorwa-Grauslund, Marie F (author), Xavier, Ana M R B (author)
Format: article
Language:eng
Published: 2017
Subjects:
Hardwood spent sulfite liquor (HSSL)
Scheffersomyces stipitis
Lignocellulosic inhibitors
Evolutionary engineering
Bioethanol
Online Access:http://hdl.handle.net/10773/18152
Country:Portugal
Oai:oai:ria.ua.pt:10773/18152
Description
Summary:Hardwood spent sulfite liquor (HSSL) is a by-product of acid sulfite pulping process that is rich in xylose, a monosaccharide that can be fermented to ethanol by Scheffersomyces stipitis. However, HSSL also contains acetic acid and lignosulfonates that are inhibitory compounds of yeast growth. The main objective of this study was the use of an evolutionary engineering strategy to obtain variants of S. stipitis with increased tolerance to HSSL inhibitors while maintaining the ability to ferment xylose to ethanol.

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