Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria
Fluorescence in situ Hybridization (FISH) is a versatile, widespread and widely-used technique in microbiology. The first step of FISH fixation/permeabilization is crucial to the outcome of the method. This work aimed to systematically evaluate fixation/permeabilization protocols employing ethanol,...
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| Other Authors: | , |
| Format: | article |
| Language: | eng |
| Published: |
2018
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| Online Access: | https://hdl.handle.net/10216/115925 |
| Country: | Portugal |
| Oai: | oai:repositorio-aberto.up.pt:10216/115925 |