Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris

In Saccharomyces cerevisiae the activity for the lactate proton-symporter in dependent on JEN1 gene expression. Pichia pastoris was transformed with an integrative plasmid containing the JEN1 gene. After 24 h of methanol induction, Northern and Western-blotting analyses indicated the expression of J...

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Bibliographic Details
Main Author: Silva, Isabel Soares (author)
Other Authors: Schuller, Dorit Elisabeth (author), Andrade, Raquel P. (author), Baltazar, Fátima (author), Cássio, Fernanda (author), Casal, Margarida (author)
Format: article
Language:eng
Published: 2003
Subjects:
Yeast
JEN1
Lactate transporter
Heterologous expression
Pichia pastoris
Functional reconstitution
Science & Technology
Online Access:http://hdl.handle.net/1822/2218
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/2218

Internet

http://hdl.handle.net/1822/2218

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