The spoilage yeast Zygosaccharomyces bailii forms mitotic spores : a screening method for haploidization

Zygosaccharomyces bailii ISA 1307 and the type strain of this spoilage yeast show a diploid DNA content. Together with a rather peculiar life cycle in which mitotic but no meiotic spores appear to be formed, the diploid DNA content explains the observed difficulties in obtaining auxotrophic mutants....

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Bibliographic Details
Main Author: Rodrigues, Fernando José dos Santos (author)
Other Authors: Ludovico, Paula (author), Sousa, Maria João (author), Steensma, H. Yde (author), Côrte-Real, Manuela (author), Leão, Cecília (author)
Format: article
Language:eng
Published: 2003
Subjects:
Yeast
Mytotic spores
Science & Technology
Online Access:http://hdl.handle.net/1822/1711
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/1711
Description
Summary:Zygosaccharomyces bailii ISA 1307 and the type strain of this spoilage yeast show a diploid DNA content. Together with a rather peculiar life cycle in which mitotic but no meiotic spores appear to be formed, the diploid DNA content explains the observed difficulties in obtaining auxotrophic mutants. Mitotic chromosome loss induced by benomyl and selection on canavanine media resulted in three haploid strains of Z. bailii. This new set of Z. bailii strains allows the easy isolation of recessive mutants and is suitable for further molecular genetic studies.

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