High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures

Silk-elastin-like polymers (SELPs) are protein-based polymers composed of repetitive amino acid sequence motifs found in silk fibroin (GAGAGS) and mammalian elastin (VPGVG). These polymers are of much interest, both from a fundamental and applied point of view, finding potential application in biome...

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Bibliographic Details
Main Author: Machado, Raul (author)
Other Authors: Azevedo-Silva, João (author), Correia, Cristina (author), Collins, Tony (author), Arias, Francisco Javier (author), Rodríguez-Cabello, Jose Carlos (author), Casal, Margarida (author)
Format: article
Language:eng
Published: 2013
Subjects:
Silk-elastin-like polymers
Recombinant protein expression
Auto induction
Acid-based cell lysis
Ammonium sulphate purification
Non-chromatographic purification
Ciências Naturais::Ciências Biológicas
Science & Technology
Online Access:http://hdl.handle.net/1822/45776
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/45776

Internet

http://hdl.handle.net/1822/45776

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