Reversible and Selective Interconversion of Hydrogen and Carbon Dioxide into Formate by a Semiartificial Formate Hydrogenlyase Mimic

The biological formate hydrogenlyase (FHL) complex links a formate dehydrogenase (FDH) to a hydrogenase (H2ase) and produces H2 and CO2 from formate via mixed-acid fermentation in Escherichia coli. Here, we describe an electrochemical and a colloidal semiartificial FHL system that consists of an FDH...

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Bibliographic Details
Main Author: Sokol, Katarzyna P. (author)
Other Authors: Robinson, William E. (author), Oliveira, Ana R. (author), Zacarias, Sonia (author), Lee, Chong Yong (author), Madden, Christopher (author), Bassegoda, Arnau (author), Hirst, Judy (author), Pereira, Inês A.C. (author), Reisner, Erwin (author)
Format: article
Language:eng
Published: 2020
Subjects:
Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry
Online Access:http://hdl.handle.net/10362/91347
Country:Portugal
Oai:oai:run.unl.pt:10362/91347
Description
Summary:The biological formate hydrogenlyase (FHL) complex links a formate dehydrogenase (FDH) to a hydrogenase (H2ase) and produces H2 and CO2 from formate via mixed-acid fermentation in Escherichia coli. Here, we describe an electrochemical and a colloidal semiartificial FHL system that consists of an FDH and a H2ase immobilized on conductive indium tin oxide (ITO) as an electron relay. These in vitro systems benefit from the efficient wiring of a highly active enzyme pair and allow for the reversible conversion of formate to H2 and CO2 under ambient temperature and pressure. The hybrid systems provide a template for the design of synthetic catalysts and surpass the FHL complex in vivo by storing and releasing H2 on demand by interconverting CO2/H2 and formate with minimal bias in either direction.

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