Metagenomics approach to unravel the potential of lignocellulosicresidues towards the discovery of novel enzymes

Composting units which handle lignocellulosic residues are suitable sources of novel and promising lignocellulose-degrading enzymes such as cellulases, xylanases and laccases. These enzymes have practical application in biorefineries where lignocellulose is converted into several added-value bioprod...

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Bibliographic Details
Main Author: Santos, A. O. (author)
Other Authors: Costa, Ângela Maria Araújo (author), Sousa, Joana Raquel Fernandes (author), Gudiña, Eduardo José (author), Rodrigues, Joana Lúcia Lima Correia (author), Silvério, Sara Isabel Cruz (author), Rodrigues, Lígia R. (author)
Format: conferenceObject
Language:eng
Published: 2021
Subjects:
Metagenomics
Online Access:http://hdl.handle.net/1822/76769
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/76769
Description
Summary:Composting units which handle lignocellulosic residues are suitable sources of novel and promising lignocellulose-degrading enzymes such as cellulases, xylanases and laccases. These enzymes have practical application in biorefineries where lignocellulose is converted into several added-value bioproducts. However, the effective conversion of lignocellulose by a sustainable process is currently incomplete and there is a need to find novel and robust catalysts to overcome this fact. A function-based metagenomic approach was performed to identify novel lignocellulose-degrading enzymes. The DNA was extracted from compost samples and fosmid metagenomic libraries were constructed. A functional two-step screening was performed to select the most promising catalytic actions under extreme pH and temperature conditions.

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