Evidence of vertical transmission of ostreid herpesvirus 1 in the Portuguese oyster Crassostrea angulata
In order to determine if ostreid herpesvirus 1 (OsHV-1) can be vertically transmitted, 9 full-sib families of the Portuguese oyster Crassostrea angulata were produced using a factorial mating design with 3 males and 3 females. The parents were survivors from an OsHV-1 mortality outbreak. OsHV-1 DNA...
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| Other Authors: | , , , |
| Format: | article |
| Language: | eng |
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2017
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10400.1/9266 |
| Country: | Portugal |
| Oai: | oai:sapientia.ualg.pt:10400.1/9266 |
| Summary: | In order to determine if ostreid herpesvirus 1 (OsHV-1) can be vertically transmitted, 9 full-sib families of the Portuguese oyster Crassostrea angulata were produced using a factorial mating design with 3 males and 3 females. The parents were survivors from an OsHV-1 mortality outbreak. OsHV-1 DNA was not detected by conventional PCR in the mantle of parents, gametes or 3 day-old larvae. However, viral DNA was detected by real-time PCR in all gametes and larvae samples. These results show that C. angulata that have survived an OsHV-1 mortality outbreak can carry the virus and vertically transmit it to their offspring. (C) 2016 Elsevier Inc. All rights reserved. |
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