A rapid and inexpensive molecular technique to discriminate the north-eastern Atlantic and Mediterranean Atherina species and its potential applications in ecology and larval identification

This note describes a rapid and inexpensive Restriction Fragment Length Polymorphism technique to discriminate all species of Atherina (Pisces: Atherinidae) of the north-eastern Atlantic and the Mediterranean. This technique is based on digestion of a fragment of the 12S ribosomal RNA (12SrRNA) gene...

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Bibliographic Details
Main Author: Almada, Vítor Carvalho (author)
Other Authors: Francisco, Sara Martins (author), Rosa, Inês (author), Domingues, Vera dos Santos (author), Cabral, Henrique (author), Almada, Frederico José Oliveira de (author), Henriques, Miguel (author), Robalo, Joana Isabel (author)
Format: article
Language:eng
Published: 2012
Subjects:
Atherina
RFLP
mtDNA
Online Access:http://hdl.handle.net/10400.12/1791
Country:Portugal
Oai:oai:repositorio.ispa.pt:10400.12/1791
Description
Summary:This note describes a rapid and inexpensive Restriction Fragment Length Polymorphism technique to discriminate all species of Atherina (Pisces: Atherinidae) of the north-eastern Atlantic and the Mediterranean. This technique is based on digestion of a fragment of the 12S ribosomal RNA (12SrRNA) gene region of mitochondrial DNA with restriction enzymes that recognize species-specific nucleotide sites. The three currently recognized species in the area, as well as two additional forms awaiting formal description, can be discriminated using a set of four endonucleases. We argue that this simple and fast technique may be of great help in the identification of young stages and in ecological surveys.

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