Direct single-step surface plasmon resonance analysis of interactions between small peptides and immobilized monoclonal antibodies
Surface plasmon resonance (SPR) methods have been optimized to permit direct kinetic analysis of the antigenic peptide analytes interacting with immobilized monoclonal antibodies (mAbs). High reproducibility and a significant correlation between SPR and previous ELISA data on the same set of antibod...
| Autor principal: | |
|---|---|
| Outros Autores: | , |
| Formato: | article |
| Idioma: | eng |
| Publicado em: |
2000
|
| Assuntos: | |
| Texto completo: | https://hdl.handle.net/10216/82066 |
| País: | Portugal |
| Oai: | oai:repositorio-aberto.up.pt:10216/82066 |
| Resumo: | Surface plasmon resonance (SPR) methods have been optimized to permit direct kinetic analysis of the antigenic peptide analytes interacting with immobilized monoclonal antibodies (mAbs). High reproducibility and a significant correlation between SPR and previous ELISA data on the same set of antibodies and peptides were observed. The kinetic data obtained provide further insight into the structure of the main antigenic site of foot-and-mouth disease virus (FMDV). |
|---|