Ultrasound as a apid and Low-cost extraction procedure to obtain Anthocyanin-based colorants from Prunus spinosa L. Fruit epicarp: comparative study with conventional heat-based extraction

An ultrasound rapid and low-cost procedure for anthocyanin-based colorants from Prunus spinosa L. fruit epicarp was developed, and the advantages were compared with conventional heat-based extraction. To obtain the conditions that maximize anthocyanins’ extraction, a response surface methodology was...

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Detalhes bibliográficos
Autor principal: Leichtweis, Maria Gabriela (author)
Outros Autores: Pereira, Carla (author), Prieto Lage, Miguel A. (author), Barreiro, M.F. (author), Baraldi, Ilton J. (author), Barros, Lillian (author), Ferreira, Isabel C.F.R. (author)
Formato: article
Idioma:eng
Publicado em: 2018
Assuntos:
Texto completo:http://hdl.handle.net/10198/20070
País:Portugal
Oai:oai:bibliotecadigital.ipb.pt:10198/20070
Descrição
Resumo:An ultrasound rapid and low-cost procedure for anthocyanin-based colorants from Prunus spinosa L. fruit epicarp was developed, and the advantages were compared with conventional heat-based extraction. To obtain the conditions that maximize anthocyanins’ extraction, a response surface methodology was applied using the variables of time, temperature, and ethanol content, in the case of heat extraction, whereas for ultrasound assisted extraction, temperature was replaced by ultrasound power. Two anthocyanin compounds were identified by HPLC-DAD-ESI/MS—namely, cyanidin 3-rutinoside and peonidin 3-rutinoside. The responses used were the extraction yield and the content of the identified anthocyanins. Ultrasound extraction was the most effective method at 5.00 0.15 min, 400.00 32.00 W, and 47.98% 2.88% of ethanol obtaining 68.60% 2.06% of extracted residue, with an anthocyanin content of 18.17 mg/g (extract-basis) and 11.76 mg/g (epicarp-basis). Overall, a viable green process was achieved that could be used to support pilot-scale studies for industrial production of anthocyanin-based colorants from P. spinosa fruit epicarp.