Optimization of peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method for the detection of bacteria: the effect of pH, dextran sulfate and probe concentration

Fluorescence in situ hybridization (FISH) appeared in the 1980’s and is nowadays widely used in the field of microbiology. FISH is affected by a wide variety of abiotic and biotic variables and their interplay. This is translated into a wide variability of FISH procedures that can be found in the li...

Full description

Bibliographic Details
Main Author: Rocha, Rui (author)
Other Authors: Santos, R. (author), Madureira, P. (author), Almeida, Carina (author), Azevedo, N. F. (author)
Format: conferenceObject
Language:eng
Published: 2014
Online Access:http://hdl.handle.net/1822/31836
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/31836