A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease
Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capil...
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| Other Authors: | , , |
| Format: | article |
| Language: | eng |
| Published: |
2000
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| Subjects: | |
| Online Access: | http://hdl.handle.net/1822/1183 |
| Country: | Portugal |
| Oai: | oai:repositorium.sdum.uminho.pt:1822/1183 |