A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease

Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capil...

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Detalhes bibliográficos
Autor principal: Lemos, M. A. (author)
Outros Autores: Teixeira, J. A. (author), Mota, M. (author), Gama, F. M. (author)
Formato: article
Idioma:eng
Publicado em: 2000
Assuntos:
Texto completo:http://hdl.handle.net/1822/1183
País:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/1183