Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography

This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded...

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Bibliographic Details
Main Author: Moreira,Keila Aparecida (author)
Other Authors: Diogo,Margarida (author), Prazeres,Duarte Miguel (author), Lima Filho,José Luiz de (author), Porto,Ana Lúcia Figueiredo (author)
Format: article
Language:eng
Published: 2005
Subjects:
Gene therapy
hydrophobic interaction chromatography
plasmid
purification
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014
Country:Brazil
Oai:oai:scielo:S1516-89132005000400014

Internet

http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014

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