Cloning and sequencing of a full-length sea bream (Sparus aurata) beta-actin cDNA

A full-length cDNA clone encoding beta-actin (b-actin) was isolated from a sea bream (Sparus aurata) liver cDNA library. Sequencing of this clone reveals an open reading frame encoding a 375 amino acid protein that shares a high degree of conservation to other known actins. The sea breamb-actin sequ...

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Bibliographic Details
Main Author: Santos, Cecilia (author)
Other Authors: Power, Deborah (author), Kille, Peter (author), Llewellyn, Lynda (author), Ramsurn, Vimi P. (author), Wigham, Trevor (author), Sweeney, Glen E. (author)
Format: article
Language:eng
Published: 2014
Subjects:
b-actin cDNA
Conservation
Evolution
Sea bream (Sparus aurata)
Teleost
Online Access:http://hdl.handle.net/10400.1/5450
Country:Portugal
Oai:oai:sapientia.ualg.pt:10400.1/5450
Description
Summary:A full-length cDNA clone encoding beta-actin (b-actin) was isolated from a sea bream (Sparus aurata) liver cDNA library. Sequencing of this clone reveals an open reading frame encoding a 375 amino acid protein that shares a high degree of conservation to other known actins. The sea breamb-actin sequence showed 98% identity to carp and human b-actin and 95% and 94% identity to sea squirt and Dictyostelium cytoplasmic actins, respectively.

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