A cell-free organelle-based in vitro system for studying the peroxisomal protein import machinery
Here we describe a protocol to dissect the peroxisomal matrix protein import pathway using a cell-free in vitro system. The system relies on a postnuclear supernatant (PNS), which is prepared from rat/mouse liver, to act as a source of peroxisomes and cytosolic components. A typical in vitro assay c...
| Main Author: | |
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| Other Authors: | , , , , |
| Format: | article |
| Language: | eng |
| Published: |
2016
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| Online Access: | https://repositorio-aberto.up.pt/handle/10216/117921 |
| Country: | Portugal |
| Oai: | oai:repositorio-aberto.up.pt:10216/117921 |