Mycobacterium tuberculosis genetic diversity and drug resistance across Portuguese-speaking countries and CPLP-TB: a novel framework and surveillance tool for the Lusophone community

Background: Tuberculosis (TB) remains a major health problem within the Community of Portuguese Language Speaking Countries (CPLP). Despite the marked variation in TB incidence across its member-states and continued human migratory flux between countries, a considerable gap in the knowledge on the M...

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Bibliographic Details
Main Author: Perdigão, João (author)
Other Authors: Silva, Carla (author), Diniz, Jaciara (author), Pereira, Catarina (author), Machado, Diana (author), Ramos, Jorge (author), Silva, Hugo (author), Abilleira, Fernanda (author), Brum, Clarice (author), Reis, Ana J. (author), Macedo, Maíra (author), Scaini, João L. (author), Silva, Ana B. (author), Esteves, Leonardo (author), Macedo, Rita (author), Maltez, Fernando (author), Clemente, Sofia (author), Coelho, Elizabeth (author), Viegas, Sofia (author), Rabna, Paulo (author), Rodrigues, Amabélia (author), Taveira, Nuno (author), Jordão, Luísa (author), Kritski, Afrânio (author), Lapa e Silva, José (author), Mokrousov, Igor (author), Couvin, David (author), Rastogi, Nalin (author), Couto, Isabel (author), Pain, Arnab (author), McNerney, Ruth (author), Clark, Taane G. (author), von Groll, Andrea (author), Dalla-Costa, Elis R. (author), Rossetti, Maria Lúcia (author), da Silva, Pedro E.A (author), Viveiros, Miguel (author), Portugal, Isabel (author)
Format: conferenceObject
Language:eng
Published: 2020
Subjects:
Mycobacterium Tuberculosis
Genetics
Surveillance
CPLP-TB
Infecções Respiratórias
Resistência aos Antimicrobianos
Online Access:http://hdl.handle.net/10400.18/6694
Country:Portugal
Oai:oai:repositorio.insa.pt:10400.18/6694
Description
Summary:Background: Tuberculosis (TB) remains a major health problem within the Community of Portuguese Language Speaking Countries (CPLP). Despite the marked variation in TB incidence across its member-states and continued human migratory flux between countries, a considerable gap in the knowledge on the Mycobacterium tuberculosis population structure and strain circulation between the countries still exists. Materials and Methods: To address this, we have assembled and analyzed the largest CPLP M. tuberculosis molecular and drug susceptibility dataset, comprised by a total of 1447 clinical isolates, including 423 multidrug-resistant isolates, from five CPLP countries. Genotyping analysis was carried out by 15/24 Mycobacterial Interspersed Repetitive Unit – Variable Number of Tandem Repeat (MIRU-VNTR) and Spoligotyping. Drug Susceptibility testing was performed using standardized BACTEC 960 MGIT methodology or through the resazurin microtiter assay (REMA). Results: The data herein presented reinforces Latin American and Mediterranean (LAM) strains as the hallmark of M. tuberculosis populational structure in the CPLP coupled with country-specific differential prevalence of minor clades. Moreover, using high-resolution typing by 24-loci MIRU-VNTR, six cross-border genetic clusters were detected, thus supporting recent clonal expansion across the Lusophone space. To make this data available to the scientific community and public health authorities we developed CPLP-TB (available at http://cplp-tb.ff.ulisboa.pt), an online database coupled with web-based tools for exploratory data analysis. Conclusions: As a public health tool, CPLP-TB is expected to contribute to improved knowledge on the M. tuberculosis population structure and strain circulation within the CPLP, thus supporting risk assessment of strain-specific trends.

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