Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression
In the present study, the Sparus aurata -skeletal actin was cloned from a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. The deduced amino acid sequence of sea bream -actin is identical to Fugu -a...
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| Other Authors: | , , , |
| Format: | article |
| Language: | eng |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10400.1/3951 |
| Country: | Portugal |
| Oai: | oai:sapientia.ualg.pt:10400.1/3951 |
| Summary: | In the present study, the Sparus aurata -skeletal actin was cloned from a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. The deduced amino acid sequence of sea bream -actin is identical to Fugu -actin-1. The expression of -actin was initiated at the onset of segmentation. In adult fish, -actin is expressed predominantly in white and red muscle. |
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