Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium

The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation tempe...

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Bibliographic Details
Main Author: Muñiz-Márquez, Diana B. (author)
Other Authors: Contreras, Juan C. (author), Rodríguez, Raúl (author), Mussatto, Solange I. (author), Teixeira, J. A. (author), Aguilar, Cristóbal N. (author)
Format: article
Language:eng
Published: 2016
Subjects:
Fructosyltransferase
Fructooligosaccharides
Aguamiel
Solid State Fermentation
Science & Technology
Online Access:http://hdl.handle.net/1822/42056
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/42056
Description
Summary:The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347 U/L) was obtained at 32 °C, using packing density of 0.7 g/cm3. Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.

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