Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons
Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this...
| Main Author: | |
|---|---|
| Other Authors: | , , , |
| Format: | article |
| Language: | eng |
| Published: |
2012
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/1822/20448 |
| Country: | Portugal |
| Oai: | oai:repositorium.sdum.uminho.pt:1822/20448 |
| Summary: | Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple noninvasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders |
|---|