Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria

Fluorescence in situ Hybridization (FISH) is a versatile, widespread and widely- used technique in microbiology. The first step of FISHfixation/permeabilizationis crucial to the outcome of the method. This work aimed to systematically evaluate fixation/permeabilization protocols employing ethanol, t...

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Bibliographic Details
Main Author: Rocha, Rui (author)
Other Authors: Almeida, Carina (author), Azevedo, Nuno F. (author)
Format: article
Language:eng
Published: 2018
Subjects:
Ethanol fixation
Gram positive bacteria
Bacillus cereus
Gram negative bacteria
Staphylococcus epidermidis
Fluorescent in situ hybridization
Pseudomonas fluorescens
Cell hybridization
Science & Technology
Online Access:http://hdl.handle.net/1822/55190
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/55190

Internet

http://hdl.handle.net/1822/55190

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