Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria
Fluorescence in situ Hybridization (FISH) is a versatile, widespread and widely- used technique in microbiology. The first step of FISHfixation/permeabilizationis crucial to the outcome of the method. This work aimed to systematically evaluate fixation/permeabilization protocols employing ethanol, t...
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| Outros Autores: | , |
| Formato: | article |
| Idioma: | eng |
| Publicado em: |
2018
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| Assuntos: | |
| Texto completo: | http://hdl.handle.net/1822/55190 |
| País: | Portugal |
| Oai: | oai:repositorium.sdum.uminho.pt:1822/55190 |