Application of confocal microscopy for quantification of intracellular mycobacteria in macrophages.
This chapter describes the techniques used to prepare a uniform and consistent mycobacterial culture and for the infection of macrophages in vitro. Here, protocols are described for the achievement of a certain number of single cell bacilli per macrophage. Confocal microscopy in combination with the...
| Main Author: | |
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| Other Authors: | , , |
| Format: | bookPart |
| Language: | eng |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10451/8519 |
| Country: | Portugal |
| Oai: | oai:repositorio.ul.pt:10451/8519 |