Characterization of recombinant desulfovibrio gigas ferredoxin

Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe–4S] cluster per monomer. EPR and 1H NMR data of recombinant and wild-typ...

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Bibliographic Details
Main Author: Rodrigues, Pedro (author)
Other Authors: Graça, Fernando (author), Macedo, Anjos L. (author), Moura, Isabel (author), Moura, José J. G. (author)
Format: article
Language:eng
Published: 2014
Subjects:
Ferredoxin
3Fe–4S cluster
Iron–sulfur cluster
Paramagnetic protein
Ferredoxin gene
Online Access:http://hdl.handle.net/10400.1/4361
Country:Portugal
Oai:oai:sapientia.ualg.pt:10400.1/4361

Internet

http://hdl.handle.net/10400.1/4361

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