Polyethylene glycol (PEG) molecular weight influences the ClearT2 optical clearing method for spheroids imaging by confocal laser scanning microscopy

Some fluorescence microscopies, like confocal laser scanning microscopy (CLSM), have limited penetration depth. Consequently, the visualization and imaging of 3D cell cultures, such as spheroids, can be a significant challenge. Therefore, to improve the imaging of 3D tissues, clearing methods have b...

Full description

Bibliographic Details
Main Author: Costa, Elisabete Cristina da Rocha (author)
Other Authors: Moreira, André Ferreira (author), Diogo, Duarte de Melo (author), Correia, Ilídio Joaquim Sobreira (author)
Format: article
Language:eng
Published: 2018
Subjects:
Online Access:http://hdl.handle.net/10400.6/4740
Country:Portugal
Oai:oai:ubibliorum.ubi.pt:10400.6/4740