Mini-FLOTAC for the diagnosis of Eimeria infection in goats: An alternative to McMaster

Caprine coccidiosis is responsible for remarkable economic losses. Diagnosis must therefore take into account a number of epidemiological and clinical factors supported by laboratory investigations. The detection of Eimeria oocysts and the determination of oocysts per gram of faeces (OPG) require a...

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Detalhes bibliográficos
Autor principal: Silva, L.M.R. (author)
Outros Autores: Vila-Viçosa, M.J.M. (author), Maurelli, M.P. (author), Morgoglione, M.E. (author), Cortes, H.C.E. (author), Cringoli, G. (author), Rinaldi, L. (author)
Formato: article
Idioma:por
Publicado em: 2014
Assuntos:
Texto completo:http://hdl.handle.net/10174/10660
País:Portugal
Oai:oai:dspace.uevora.pt:10174/10660
Descrição
Resumo:Caprine coccidiosis is responsible for remarkable economic losses. Diagnosis must therefore take into account a number of epidemiological and clinical factors supported by laboratory investigations. The detection of Eimeria oocysts and the determination of oocysts per gram of faeces (OPG) require a trustworthy oocyst count technique. Mini-FLOTAC is a new apparatus from the FLOTAC family, which was designed for optimal examination of faecal sample suspensions in each flotation chamber (total volume = 2 mL) and which permits a maximum magnification of 400x. Faecal samples from sixteen goats reared in an intensive goat farm were qualitatively (simple flotation) and quantitatively analyzed. A comparison between McMaster (McM) and Mini-FLOTAC was carried out, by performing 288 different readings. Eimeria arloingi, E. caprovina and E. ninakohlyakimovae were the most prevalent species. The mean OPG detected by Mini-FLOTAC was higher than the values observed with McMaster techniques (P<0.05). Mini-FLOTAC also produced coefficient of variation values similar to those resulted from McM 1 mL (17.5 versus 19.4; P>0.05) but lower than those resulted from McM 0.3 mL. Thereby, the present study suggests that the Mini-FLOTAC is a promising technique for detecting and counting Eimeria spp. in goat faeces.